Queenscliff is a small town on the Bellarine Peninsula in southern Victoria, Australia and located south of Swan Bay at the entrance to Port Phillip. It is the administrative centre for the Borough of Queenscliffe. At the 2006 census, Queenscliff had a population of 1,416.

Queenscliff is a former 1880s seaside resort now known for its Victorian era heritage and tourist industry and as one of the endpoints of the Searoad ferry to Sorrento on the Mornington Peninsula.

Originally a fishing village, Queenscliff soon became an important cargo port, servicing steamships trading in Port Philip. A shipping pilot service was established in 1841, and its two lighthouses, the High and Low Lights, were constructed in 1862-63. Queenscliff also played an important military role. Fort Queenscliff was built between 1879 and 1889, and operated as the command centre for a network of forts around the port.